Officials did not immediately say where in Florida the state's winning ticket was sold.
The prize money will be split between the three jackpot winners.
Players won $2 million each in seven states, the Multi-State Lottery Association said: Arizona, Florida, Iowa, North Carolina, New Jersey, Tennessee and Texas. Players in 23 states won $1 million each.
Excluding the jackpot, Powerball officials said players won $273.9 million worth of prize money.
Since October, the Powerball jackpot has rolled over 19 times. As of Thursday morning, the jackpot went back down to $40 million.
Officials with the Multi-State Lottery Association said they expected more than 85 percent of the possible number combinations would have been bought for the drawing.
If someone matches all the winning numbers, the jackpot would be paid in annual payments over 29 years, or the winner could opt for a lump-sum payment of $930 million. Winners have to pay 39.6 percent of the prize in federal income taxes, in addition to any state taxes.
Powerball is played in 44 states as well as the District of Columbia, U.S. Virgin Islands and Puerto Rico.